Figure 2: Dose-dependent variation in membrane permeability in thymocytes by trypan blue and fluorescence of fluorescein diacetate. Mouse thymocytes were irradiated for increasing doses (0.25–10 Gy) at 4°C followed by centrifugation at 2000 rpm. The cell pellet was incubated at 37°C after re-suspending in fresh culture medium added antibiotics and 10% fetal calf serum. After 0, 15, and 24 h of incubation (a) cells were treated with trypan blue (0 h [[INSIDE:1]], 15 h [[INSIDE:2]] and 24 h [[INSIDE:3]]), (b) fluorescence intensity of irradiated (0–6 Gy) thymocytes measured at 25°C after labeling with fluorescein diacetate for 30 min and washing by phosphate-buffered saline at 4°C
![Figure 2: Dose-dependent variation in membrane permeability in thymocytes by trypan blue and fluorescence of fluorescein diacetate. Mouse thymocytes were irradiated for increasing doses (0.25–10 Gy) at 4°C followed by centrifugation at 2000 rpm. The cell pellet was incubated at 37°C after re-suspending in fresh culture medium added antibiotics and 10% fetal calf serum. After 0, 15, and 24 h of incubation (a) cells were treated with trypan blue (0 h [[INSIDE:1]], 15 h [[INSIDE:2]] and 24 h [[INSIDE:3]]), (b) fluorescence intensity of irradiated (0–6 Gy) thymocytes measured at 25°C after labeling with fluorescein diacetate for 30 min and washing by phosphate-buffered saline at 4°C](articles/2018/9/3/images/JRadiatCancerRes_2018_9_3_119_242366_f2.jpg)